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Image Search Results
Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Immunoregulatory properties of cell free DNA
doi: 10.1007/s00018-025-05862-y
Figure Lengend Snippet: cfDNA-induced variation in gene expression. ( A ) 40 genes analyzed by Bio-Rad iQ5 analysis in LPS pre-activated PMJ2-PC macrophages treated (blue bar) or not (green bar) with the poly-C/poly-G oligonucleotide (1 µg/ml for 3 h), * p -value < 0.05 and ** p -value < 0.001; ( B ) Quantitative PCR analysis on mRNAs coding for MAPK-14 , EGR-1 , and CCL22 in LPS pre-activated PMJ2-PC macrophages treated (black bar) or not (white bar) with the poly-C/poly-G oligonucleotide (1 µg/ml for 3 h). Data are expressed as fold change (2 − ΔΔCT ) normalized with GAPDH. Data are the mean of 3 concordant experiments; Student paired T test ( C ) Network analysis using the STRING-DB software of differentially expressed genes ( EGR-1 and CCL22 ) between pre-activated PMJ2-PC macrophages treated or not with the poly-C/poly-G oligonucleotide. EGR-1 and CCL22 genes are showed in red circles
Article Snippet: A pre-designed panel of pathway-specific genes (Supplementary Table ) was used to test the expression of 40 genes with
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Software